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Journal / Biochemistry / Løsning affinitetsrensede proteinkomplekser ved…
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Biochemistry

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JoVE Journal Biochemistry
Løsning affinitetsrensede proteinkomplekser ved Blå Native PAGE og protein Korrelation Profilering
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Løsning affinitetsrensede proteinkomplekser ved Blå Native PAGE og protein Korrelation Profilering

Article DOI: 10.3791/55498-v • 09:35 min • April 1st, 2017
April 1st, 2017 •
1, 1, 1, 1
1Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute

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Vi præsenterer her protokoller for affinitetsoprensning af proteinkomplekser og deres separation ved blå nativ PAGE, efterfulgt af protein korrelation profilering ved anvendelse etiket fri kvantitativ massespektrometri. Denne metode er nyttig til at løse interactomes i distinkte proteinkomplekser.

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Biokemi Blå nativ PAGE protein korrelation profilering multiproteinkompleks affinitetsoprensning kvantitativ massespektrometri interactome AP-MS proteininteraktion
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