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Journal / Immunology and Infection / Un modelo de ratón IL-8 transgénicamente…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Un modelo de ratón IL-8 transgénicamente transgéni-
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Un modelo de ratón IL-8 transgénicamente transgéni-

Article DOI: 10.3791/55499-v • 08:16 min • July 7th, 2017
July 7th, 2017 •
*1, *2, *3, 3, 4, 5,2, 6, 7, 2, 8, 1
1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, 2Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division, University of Verona, 4Dipartimento di Scienze Medico-Veterinarie, University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences, University of Parma, 6Department of Computer Science, University of Verona, 7Department of Neurological, Biomedical and Movement Sciences, University of Verona, 8Cystic Fibrosis Regional Center (CFC), AOUI Verona
* These authors contributed equally

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El método descrito aquí permite la visualización de la activación de la inflamación dependiente del promotor de IL-8 en los pulmones de ratones a través de imágenes de bioluminiscencia no invasivas (BLI). El mismo animal puede ser sometido a BLI múltiples veces durante hasta dos meses a partir del momento de la entrega de la construcción de indicador de luciferasa.

Tags

Inmunología Número 125 Inflamación entrega de genes, bioluminiscencia interleucina-8 pulmón
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