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Optisk kvantificering af intracellulære pH i Drosophila melanogaster Malpighian tubulus epitheler med en fluorescerende genetisk kodet pH indikator
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Optisk kvantificering af intracellulære pH i Drosophila melanogaster Malpighian tubulus epitheler med en fluorescerende genetisk kodet pH indikator

Article DOI: 10.3791/55698-v • 11:54 min • August 11th, 2017
August 11th, 2017 •
1, 1,2
1Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, 2Department of Nephrology and Hypertension, Mayo Clinic College of Medicine

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Cellulære ion transport kan ofte vurderes ved at overvåge intracellulære pH (pH,jeg). Genetisk giver kodet pH-indikatorer (GEpHIs) optisk kvantificering af intracellulære pH i intakt celler. Denne protokol detaljer kvantificering af intracellulære pH via cellular ex vivo live-billeddannelse af Malpighian tubuli af Drosophila melanogaster med pHerry, en pseudo-ratiometric kodet genetisk pH-indikator.

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Biokemi sag 126 Ion transport intracellulære pH epitheler Malpighian tubuli Drosophila fluorescerende reporter genetisk kodet pH indikator pH-regulering live imaging pHerry
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