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用荧光基因编码的 ph 指示剂对果蝇氏小管上皮细胞内 ph 值的光学定量
JoVE Journal
Biology
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JoVE Journal Biology
Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator
DOI:

11:54 min

August 11, 2017

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Chapters

  • 00:05Title
  • 00:54Dissection of Adult Drosophila Anterior Malpighian Tubules
  • 04:32Validation of Imaging Protocol and Tubule Health
  • 09:47Results: Quantification of Acid Extrusion in Malpighian Tubule Epithelia
  • 10:56Conclusion

Summary

Automatic Translation

细胞离子传输通常可以通过监测细胞内 ph 值(ph 值i)来评估。基因编码的 ph 值指标(GEpHIs)提供了细胞内 ph 值的光学定量。本议定书详细介绍了细胞内 ph 值的量化, 通过蜂窝状的体活成像的氏管的果蝇与 pHerry, pseudo-ratiometric 基因编码的 pH 指标。

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