Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

Adam J. Rossano; Michael F. Romero

doi:10.3791/55698

Journal

/

Biology

/

用荧光基因编码的 ph 指示剂对果蝇氏小管上皮细胞内 ph 值的光学定量

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

Please note that all translations are automatically generated. Click here for the English version.

用荧光基因编码的 ph 指示剂对果蝇氏小管上皮细胞内 ph 值的光学定量

DOI:

10.3791/55698-v

•

11:54 min

•

August 11, 2017

•

Adam J. Rossano, Michael F. Romero²

¹Department of Physiology and Biomedical Engineering,Mayo Clinic College of Medicine, ²Department of Nephrology and Hypertension,Mayo Clinic College of Medicine

Chapters

00:05Title
00:54Dissection of Adult Drosophila Anterior Malpighian Tubules
04:32Validation of Imaging Protocol and Tubule Health
09:47Results: Quantification of Acid Extrusion in Malpighian Tubule Epithelia
10:56Conclusion

Summary

Automatic Translation

细胞离子传输通常可以通过监测细胞内 ph 值(ph 值_i)来评估。基因编码的 ph 值指标(GEpHIs)提供了细胞内 ph 值的光学定量。本议定书详细介绍了细胞内 ph 值的量化, 通过蜂窝状的前体活成像的氏管的果蝇与 pHerry, pseudo-ratiometric 基因编码的 pH 指标。