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JoVE Journal Chemistry
Integration af miniaturiseret fastfaseekstraktion og LC-MS / MS-detektion af 3-nitrotyrosin i human urin til kliniske applikationer
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Integration af miniaturiseret fastfaseekstraktion og LC-MS / MS-detektion af 3-nitrotyrosin i human urin til kliniske applikationer

Article DOI: 10.3791/55778-v • 08:41 min • July 14th, 2017
July 14th, 2017 •
1, 1, 2, 1,2
1Pharmasan Labs, Inc., 2NeuroScience, Inc.

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En selektiv og følsom væskekromatografi-tandemmassespektrometri-metode (LC-MS / MS) kombineret med en effektiv fastfaseekstraktion på en mikronpladet 96-brønds mixplast med blandet tilstand (MCX) blev udviklet til måling af frit 3-nitrotyrosin ( 3-NT) i human urin med høj gennemstrømning, som er egnet til kliniske anvendelser.

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Kemi udgave 125 3-nitrotyrosin (3-NT) væskekromatografi-tandemmassespektrometri (LC-MS / MS) fastfaseekstraktion (SPE) ikke-invasiv biomarkør oxidativ stress urin følsomhed selektivitet
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