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Journal / Chemistry / Protokol til fastfasesyntese af oligomerer af RNA …
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JoVE Journal Chemistry
Protokol til fastfasesyntese af oligomerer af RNA indeholdende en 2'-
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Protokol til fastfasesyntese af oligomerer af RNA indeholdende en 2'-

Article DOI: 10.3791/56189-v • 11:37 min • July 28th, 2017
July 28th, 2017 •
1, 1
1Department of Chemistry, University of Colorado Denver

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Denne artikel tilvejebringer en detaljeret fremgangsmåde til fastfasesyntese, oprensning og karakterisering af dodecamerer af RNA modificeret ved C2'- O- stillingen. UV-vis og cirkulær dikroisme fotometriske analyser bruges til at kvantificere og karakterisere strukturelle aspekter, dvs. enkeltstrenger eller dobbeltstrenger.

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Biochemistry RNA fastfasesyntese RNA-cirkulær dichroisme modificeret RNA oprensning af RNA massespektrometri af RNA syntetiske oligonukleotider.
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