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JoVE Journal Neuroscience
Forbedre anvendelsen av høy molekylvekt Biotinylated dekstran Amin for Thalamocortical projeksjon sporing i rotte
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Forbedre anvendelsen av høy molekylvekt Biotinylated dekstran Amin for Thalamocortical projeksjon sporing i rotte

Article DOI: 10.3791/55938-v • 06:39 min • April 12th, 2018
April 12th, 2018 •
*1, *1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

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Her presenterer vi en raffinert protokoll for å effektivt avsløre biotinylated dekstran Amin (BDA) merking med et fluorescerende flekker metoden ved hjelp av en gjensidig nevrale sti. Det er egnet for analyse av fine strukturen av BDA merking og skille den fra andre nevrale elementer under en AC confocal laserskanning mikroskop.

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Nevrovitenskap problemet 134 Biotinylated dekstran Amin tracer neural merking axon Nevron somatosensory cortex
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