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JoVE Journal Biochemistry
En Guide til produktion, krystallisering og struktur bestemmelse af menneskelige IKK1/α
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En Guide til produktion, krystallisering og struktur bestemmelse af menneskelige IKK1/α

Article DOI: 10.3791/56091-v • 11:27 min • November 2nd, 2018
November 2nd, 2018 •
1,2, 1, 1, 1
1Department of Chemistry & Biochemistry, University of California, San Diego, 2Department of Biophysics, Bose Institute

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IκB Kinase 1/α (IKK1/α CHUK) er en Ser/Thr protein kinase, der er involveret i et utal af cellulære aktiviteter primært gennem aktivering af NF-κB transkriptionsfaktorer. Her beskriver vi de vigtigste trin er nødvendige for produktionen og Krystalstrukturbestemmelse af dette protein.

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Biokemi sag 141 Serin-Threonin protein Kinase IKK1/α NF-κB krystalstruktur Molekylær udskiftning hæmmer
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