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Biochemistry

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A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
 

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Article doi: 10.3791/56091
November 2nd, 2018

Summary November 2nd, 2018

IκB Kinase 1/α (IKK1/α CHUK) is a Ser/Thr protein kinase that is involved in a myriad of cellular activities primarily through activation of NF-κB transcription factors. Here, we describe the main steps necessary for the production and crystal structure determination of this protein.

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