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Immunology and Infection
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Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis

Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis

DOI:
10.3791/56123-v

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06:09 min

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September 30, 2017

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, , , , , ,
1South Texas Center for Emerging Infectious Diseases,University of Texas San Antonio, 2Midwestern University

Chapters

  • 00:05Title
  • 01:36Prepare Catheter and Trachea Indicator
  • 02:22Intratracheal Inoculation
  • 04:02Results: Immune Response in Rats to F. tularensis
  • 04:47Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes intratracheal inoculations of Fischer 344 rats with Francisella tularensis. This procedure mimics pulmonary exposure of humans to this potential biothreat agent and can be used to test vaccine and therapeutic efficacy against pulmonary tularemia.

Tags

Intratracheal InoculationFischer 344 RatsFrancisella TularensisPulmonary TularemiaVaccine EfficacyBiothreat AgentPulmonary Infectious Disease ModelPlagueCatheterTracheaEsophagusAnesthesiaLaryngoscope

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