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A longo prazo dispositivo de imagens ao vivo para melhor manipulação Experimental de larvas de Zebrafish

Article DOI: 10.3791/56340-v • 14:05 min • October 27th, 2017
October 27th, 2017 •
*1,2, *3, 2, 2, 4, 5,6, 1,2,3
1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Morgridge Institute for Research, University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 6Department of Pediatrics, University of Wisconsin-Madison
* These authors contributed equally

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Este manuscrito descreve o zWEDGI (zebrafish Wounding e dispositivo de uma armadilha para o crescimento e a geração de imagens), que é um dispositivo compartimentado, projetado para orientar e restringir as larvas zebrafish. O projeto permite a transecção de cauda e a longo prazo coleção de imagens de alta resolução de microscopia fluorescente de cicatrização e regeneração.

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Ferida de bioengenharia edição 128 cura zebrafish microscopia do multiphoton dispositivo de retenção larvas a longo prazo de imagens
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