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Journal
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Bioengineering
/
改善斑马鱼幼虫实验操作的长期活体成像装置
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae
Please note that all translations are automatically generated.
Click here for the English version.
改善斑马鱼幼虫实验操作的长期活体成像装置
DOI:
10.3791/56340-v
•
14:05 min
•
October 27, 2017
•
Kayla Huemer*
1,2
,
Jayne M. Squirrell*
3
,
Robert Swader
,
Kirsten Pelkey
,
Danny C. LeBert
,
Anna Huttenlocher
6
,
Kevin W. Eliceiri
2,3
1
Department of Biomedical Engineering
,
University of Wisconsin-Madison
,
2
Morgridge Institute for Research
,
University of Wisconsin-Madison
,
3
Laboratory for Optical and Computational Instrumentation
,
University of Wisconsin-Madison
,
4
Cellular and Molecular Pathology Graduate Program
,
University of Wisconsin-Madison
,
5
Department of Medical Microbiology and Immunology
,
University of Wisconsin-Madison
,
6
Department of Pediatrics
,
University of Wisconsin-Madison
Chapters
00:05
Title
00:57
Design and 3D Printing of Molds
02:57
PDMS Fabrication of zWEDGI Device
04:43
Plasma-bonding zWEDGI to Glass Dish
07:19
Channel Preparation and Loading Larvae
09:52
Wounding and Imaging Larvae and End of Experiment
11:14
Results: Post Wound Live Imaging and 3D Reconstruction of Zebrafish Larvae Tail Fin
12:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这份手稿描述了 zWEDGI (斑马鱼伤人和诱捕装置的增长和成像), 这是一个条块分割的装置, 旨在定位和抑制斑马鱼幼虫。该设计允许尾部横断面和长期收集高分辨率的荧光显微图像的伤口愈合和再生。
Tags
Zebrafish Larvae
Wound Healing
Live Imaging
PDMS
3D Printing
Microscopic Analysis
Collagen Fiber Reorganization
Cellular Responses
ZWEDGI
PDMS Fabrication
Mold Cleaning
UV Postcuring
Sandpaper
Ultrasonic Cleaning
Isopropyl Alcohol
PDMS Casting
Vacuum Degassing
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