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JoVE Journal Cancer Research
Multiplexado de análisis de la inmunofluorescencia y la cuantificación de Intratumoral PD-1+ + de Tim-3 CD8 células+ T
Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice
Journal (Cancer Research)
Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice
Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
Journal (Cancer Research)
Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
 
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Multiplexado de análisis de la inmunofluorescencia y la cuantificación de Intratumoral PD-1+ + de Tim-3 CD8 células+ T

Article DOI: 10.3791/56606-v • 09:32 min • February 8th, 2018
February 8th, 2018 •
1, 2,3,4, 2,3, 2,3, 2,3,5, 6, 7, 2, 2, 2, 2,3,6, 8, 2,3, 4, 2,6, *4, *2,4
1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou
* These authors contributed equally

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Estudio de microambiente tumoral puede identificar biomarcadores pronósticos o predictivos de la respuesta clínica a la inmunoterapia. Presentado aquí, es un método innovador basado en situ de la fluorescencia multiespectral de imágenes para analizar y contar automáticamente varias subpoblaciones de CD8+ T las células. Esta técnica reproducible y confiable es adecuada para análisis de grandes cohortes.

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Inmunología número 132 Multiplex en situ imágenes multiespectrales técnica de inmunofluorescencia imagen digital carcinoma de células renales Tim 3 PD-1 microambiente tumoral inhibidores de checkpoint en situ el análisis de células individuales
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