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Trasplante de tumores para evaluar la dinámica de las células T CD8+ infiltrantes de tumores en ratones
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1Institute of Immunology,Third Military Medical University, 2Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital,Sun Yat-Sen University, 3Shigatse Branch, Xinqiao Hospital,Third Military Medical University, 4Department of Emergency Medicine, Southwest Hospital,Third Military Medical University, 5Cancer Center,The General Hospital of Western Theater Command
Chapters
- 00:04Introduction
- 00:56Ectopic Tumor Implantation of B16F10-OVA Cells in the Inguinal Region of Mice
- 02:23Adoptive Transfer of Congenically Marked OT-I T Cells into Tumor-Bearing Mice
- 03:26Dissect Tumor Mass from Tumor-Bearing Donor Mice
- 04:31Subcutaneous Transplantation of Donor-Derived Tumor onto the Tumor-Matched Recipient Mice
- 06:14Results: For Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
- 07:04Conclusion
Aquí, presentamos un protocolo de trasplante tumoral para la caracterización de linfocitos infiltrados tumorales inherentes y derivados de la periferia en un modelo de tumor de ratón. El rastreo específico de la afluencia de células inmunes derivadas del receptor con citometría de flujo revela la dinámica de los cambios fenotípicos y funcionales de estas células durante las respuestas inmunes antitumorales.
