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JoVE Journal Biochemistry
Método para dobrar eficiente e purificação do Chemoreceptor Ligand Binding Domain
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Método para dobrar eficiente e purificação do Chemoreceptor Ligand Binding Domain

Article DOI: 10.3791/57092-v • 14:25 min • December 12th, 2017
December 12th, 2017 •
1,2, 1,2,3
1Infection and Immunity Program, Monash Biomedicine Discovery Institute, 2Department of Microbiology, Monash University, 3Department of Biochemistry and Molecular Biology, Monash University

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Um procedimento é apresentado para o dobrar do domínio de ligação do ligante periplasmático dCACHE de Campylobacter jejuni chemoreceptor Tlp3 de corpos de inclusão e a purificação para produzir quantidades de miligrama de proteína.

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Bioquímica edição 130 quimiotaxia chemoreceptor transdutor como proteína sensoriamento domínio ligante dCACHE dobrar
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