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Método para dobrar eficiente e purificação do Chemoreceptor Ligand Binding Domain
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
DOI:

14:25 min

December 12, 2017

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Chapters

  • 00:05Title
  • 00:46Expression of His6-TIp3-LBD in E. coli
  • 02:29Isolation and Denaturation of Inclusion Bodies
  • 05:34Protein Refolding
  • 07:39Purification of His6-tagged Protein Using Immobilized Metal Ion Affinity Chromatogrphy
  • 08:49His6-tag Removal Using TEV Protease and Size-excluson Chromatography of Tlp3-LBD
  • 11:06Results: Expression, Refolding, and Purification of Tlp3-LBD
  • 12:41Conclusion

Summary

Automatic Translation

Um procedimento é apresentado para o dobrar do domínio de ligação do ligante periplasmático dCACHE de Campylobacter jejuni chemoreceptor Tlp3 de corpos de inclusão e a purificação para produzir quantidades de miligrama de proteína.

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