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JoVE Journal Neuroscience
Isolasjon og kultur av gnager Microglia å fremme en dynamisk Ramified morfologi i Serum-free Medium
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Isolasjon og kultur av gnager Microglia å fremme en dynamisk Ramified morfologi i Serum-free Medium

Article DOI: 10.3791/57122-v • 12:00 min • March 9th, 2018
March 9th, 2018 •
1, 1
1Department of Neurobiology, Stanford University

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Innsats for å forstå microglial funksjonen i detalj har blitt hindret av mangelen på microglial kultur modeller som recapitulate egenskapene for eldre i vivo microglia. Denne protokollen beskriver isolasjon og kultur tilnærming beregnet på vedlikeholde robuste overlevelse av svært ramified modent rotta microglia under definert mellomstore forhold.

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Nevrovitenskap problemet 133 Microglia celle kultur definert Medium nevrovitenskap Macrophage Serum-Free Ramified immunologi
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