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Chapters
- 00:04Title
- 01:25Setting Up rec-8 RNAi (Day 1 – 3 in Figure 2)
- 02:32Generating and Isolating Tetraploids (Day 4 – 16 in Figure 2)
- 03:57Verifying Tetraploid Strains using DAPI Staining
- 05:56Results: Generation of Tetraploid C. elegans Strains
- 07:11Conclusion
This method allows for the generation of tetraploid and triploid Caenorhabditis nematodes from any diploid strain. Polyploid strains generated by this method have been used to study chromosome interactions in meiotic prophase, and this method is useful for examining important basic questions in cell, developmental, evolutionary, and cancer biology.
