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Journal / Developmental Biology / CRISPR-gemedieerde verlies van functie analyse in …
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Developmental Biology

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JoVE Journal Developmental Biology
CRISPR-gemedieerde verlies van functie analyse in cerebellaire submodule cellen gebruiken In Utero Electroporation gebaseerde gentransfer
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CRISPR-gemedieerde verlies van functie analyse in cerebellaire submodule cellen gebruiken In Utero Electroporation gebaseerde gentransfer

Article DOI: 10.3791/57311-v • 09:39 min • June 9th, 2018
June 9th, 2018 •
*1, *2, 2, 3,4,5, 1, 3,4
1Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology, Heidelberg University Hospital
* These authors contributed equally

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Conventionele studies van de verlies-van-functie van genen met behulp van knock-out dieren zijn vaak kostbare en tijdrovende geweest. Electroporation gebaseerde CRISPR-gemedieerde somatische mutagenese is een krachtig hulpmiddel om te begrijpen gen functioneert in vivo. Wij rapporteren hier een methode voor het analyseren van de knock-out fenotypen in delende cellen van het cerebellum.

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Ontwikkelingsbiologie kwestie 136 gerichte gene levering in utero electroporation cerebellum GNP Cre CRISPR/Cas9 ontwikkeling van de hersenen hersentumoren
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