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Immunology and Infection

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JoVE Journal Immunology and Infection
En Protein Microarray analysen for serologisk fastsettelse av Antigen-spesifikke antistoffer svar følgende Clostridium difficile infeksjon
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En Protein Microarray analysen for serologisk fastsettelse av Antigen-spesifikke antistoffer svar følgende Clostridium difficile infeksjon

Article DOI: 10.3791/57399-v • 09:12 min • June 15th, 2018
June 15th, 2018 •
1,2, 1,2, 3
1Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen's Medical Centre, University of Nottingham, 2Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, 3Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen's Medical Centre, University of Nottingham

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Denne artikkelen beskriver en enkel protein microarray metode for profilering humoral immunreaksjoner til et 7-plex panel av svært renset Clostridium difficile antigener i menneskelig sera. Protokollen kan utvides for fastsettelse av spesifikt antistoff svar i forberedelsene til polyklonale intravenøs immunglobulin.

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Immunologi og infeksjon problemet 136 Protein microarray antistoff Clostridium difficile intravenøs immunglobulin
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