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Journal / Immunology and Infection / En rensing og I Vitro aktivitet analysen for en…
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Immunology and Infection

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JoVE Journal Immunology and Infection
En rensing og I Vitro aktivitet analysen for en (p) ppGpp Synthetase fra Clostridium difficile
Zika Virus Specific Diagnostic Epitope Discovery
Journal (Immunology and Infection)
Zika Virus Specific Diagnostic Epitope Discovery
Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies (Video) | JoVE
Journal (Immunology and Infection)
Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies (Video) | JoVE
 
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En rensing og I Vitro aktivitet analysen for en (p) ppGpp Synthetase fra Clostridium difficile

Article DOI: 10.3791/58547-v • 09:53 min • November 3rd, 2018
November 3rd, 2018 •
1, 1, 1
1Department of Chemistry and Biochemistry, Old Dominion University

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Her beskriver vi en metode for rensing histidin-merket pyrophosphokinase enzymer og utnytte tynt lag kromatografi radiolabelled underlag og produkter til analysen for den enzymatiske aktiviteten i vitro. Enzym aktivitet analysen er bredt aktuelt noen kinase, nucleotide cyclase eller fosfor-transfer reaksjon som mekanisme inkluderer nukleotid trifosfat hydrolyse.

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Immunologi og infeksjon utstede 141: Protein rensing histidin merke (p) ppGpp tynt lag Ture radionuklidenes merking pyrophosphokinase phosphotransfer
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