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Journal / Genetics / La secuencia retrospectiva MicroRNA: ADN…
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JoVE Journal Genetics
La secuencia retrospectiva MicroRNA
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La secuencia retrospectiva MicroRNA: ADN complementario biblioteca preparación protocolo utilizando a muestras de ARN de parafina-encajado formalina-fijo

Article DOI: 10.3791/57471-v • 12:24 min • May 5th, 2018
May 5th, 2018 •
1,2,3, 1,2, 3, 4
1Department of Research, Hackensack University Medical Center, 2Department of Medical Sciences, Seton Hall University, 3Department of Epidemiology and Population Health, Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

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Muestras de parafina-encajado formalina-fijos representan una valiosa fuente de biomarcadores moleculares de las enfermedades humanas. Aquí presentamos un protocolo de preparación Biblioteca de cDNA en laboratorio, inicialmente diseñado con ARN congelado fresco y optimizado para el análisis de los microRNAs de tejidos almacenan hasta 35 años.

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Genética número 135 parafina-encajado FFPE RNA RNA degradado microRNA generación de secuenciación biblioteca de cDNA cuantificación amplificación por PCR ligadura del RNA T4 página degrada el RNA formalina-fijo
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