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Chapters
- 00:04Title
- 00:393′ Barcoded Adapter Ligations with 18 Individual RNA Samples
- 02:00Ligated Small RNA Purification
- 05:265′ Ligated and 3′ Barcoded Purified Small RNAs Reverse Transcription
- 08:12Pilot PCR and Large-scale PCR Amplification
- 10:46Results: Protocol Robust Whether Fresh, Frozen, or Aged
- 12:01Conclusion
Formalin-fast parafin-embedded prøver representerer en verdifull kilde til molekylær biomarkers menneskelige sykdommer. Her presenterer vi en laboratorie-baserte cDNA biblioteket forberedelse protokoll, opprinnelig designet med fersk frosne RNA og optimalisert for analyse av arkiverte microRNAs fra vev lagret opp til 35 år.
