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Journal / Genetics / Retrospektiv mikroRNA sekvensering:…
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Genetics

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JoVE Journal Genetics
Retrospektiv mikroRNA sekvensering
An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
Journal (Genetics)
An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
Journal (Genetics)
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
 
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Retrospektiv mikroRNA sekvensering: Kompletterande DNA bibliotek förberedelse protokoll använder Formalin-fast Paraffin-inbäddat RNA exemplar

Article DOI: 10.3791/57471-v • 12:24 min • May 5th, 2018
May 5th, 2018 •
1,2,3, 1,2, 3, 4
1Department of Research, Hackensack University Medical Center, 2Department of Medical Sciences, Seton Hall University, 3Department of Epidemiology and Population Health, Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

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Formalin-fast paraffin-inbäddat exemplar representerar en värdefull källa av molekylära markörer av mänskliga sjukdomar. Här presenterar vi ett laboratoriebaserade cDNA bibliotek förberedelse protokoll, ursprungligen utformad med färsk fryst RNA och optimerad för analysen av arkiverade mikroRNA från vävnader lagras upp till 35 år.

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Genetik fråga 135 Formalin-fast paraffin-inbäddat FFPE RNA försämrade RNA mikroRNA nästa generations sekvensering cDNA bibliotek kvantifiering PCR-amplifiering T4 RNA ligatur sida försämrad RNA
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