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ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data
DOI:

05:12 min

January 16, 2019

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Chapters

  • 00:04Title
  • 00:24t-SNE Distributed Stochastic Neighbor Embedding (SNE)
  • 01:30Cluster Analysis, Filtration, and Visualization
  • 03:22Results: Representative Recapitulation of Myeloid and Lymphoid Sub-Population Data
  • 04:53Conclusion

Summary

Automatic Translation

ExCYT is a MATLAB-based Graphical User Interface (GUI) that allows users to analyze their flow cytometry data via commonly employed analytical techniques for high-dimensional data including dimensionality reduction via t-SNE, a variety of automated and manual clustering methods, heatmaps, and novel high-dimensional flow plots.

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