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Immunology and Infection
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Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore

Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore

DOI:
10.3791/60525-v

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06:01 min

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December 12, 2019

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, , ,
1Department of Immunohematology and Blood Transfusion,Leiden University Medical Center, 2Department of Parasitology,Leiden University Medical Center

Chapters

  • 00:04Title
  • 00:49Creating Clusters
  • 03:30Post-processing Clustering Analysis
  • 04:18Results: Cytofast Analysis
  • 05:26Conclusion

Summary

Automatic Translation

Automatic Translation

Cytofast is a visualization tool used to analyze output from clustering. Cytofast can be used to compare two clustering methods: FlowSOM and Cytosplore. Cytofast can rapidly generate a quantitative and qualitative overview of mass cytometry data and highlight the main differences between different clustering algorithms.

Tags

Keywords: Cytometry DataCytofastFlowSOMCytosploreHigh-dimensional DataVisualizationQuantificationCell SubsetsImmunological PatternsClinical TreatmentExperimental GroupsClusteringFCS FilesHSNETSNEMetadataFlowSet

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