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Journal / Bioengineering / 非规范氨基酸选择性压力合成抗菌肽的研究…
JoVE Journal
Bioengineering

This content is Open Access.

JoVE Journal Bioengineering
非规范氨基酸选择性压力合成抗菌肽的研究
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非规范氨基酸选择性压力合成抗菌肽的研究

Article DOI: 10.3791/57551-v • 11:56 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 3, 3, 2, 1
1Department of Biocatalysis, Institute of Chemistry, Technische Universität Berlin, 2Department of Bioenergetics, Institute of Chemistry, Technische Universität Berlin, 3Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Department of Molecular Genetics, University of Groningen
* These authors contributed equally

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该协议将非规范氨基酸 (ncAAs) 的选择性压力纳入 lactococcal 抗菌肽乳酸杆菌中。它的属性可以在重组表达式通过替换过程中通过所定义的增长媒体中所需的 ncAAs 进行更改。生物活性的变化由生长抑制试验和荧光显微学来绘制。

Tags

生物工程 135 期 乳酸链球菌素 RiPPs 安培 ncAAs lantibiotic 脯氨酸模拟 新型抗生素 活性测定 革兰氏阳性菌 重组生产 翻译后改性 合成生物学
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