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Bioengineering
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用利穆鲁布莱巴细胞裂解液 (lal) 法检测纳米配方中的内毒素
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JoVE Journal
Bioengineering
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
Please note that all translations are automatically generated.
Click here for the English version.
用利穆鲁布莱巴细胞裂解液 (lal) 法检测纳米配方中的内毒素
DOI:
10.3791/58830-v
•
06:15 min
•
January 30, 2019
•
Barry W. Neun
,
Marina A. Dobrovolskaia
1
Nanotechnology Characterization Lab., Cancer Research Technology Program
,
Frederick National Laboratory for Cancer Research sponsored by National Cancer Institute
Chapters
00:04
Title
00:32
Turbidity Limulus Amoebocyte Lysate (LAL) Assay
02:26
Sample Loading and Preparation and Gel-Clot LAL
04:27
Results: Representative Detection of Endotoxin in PEGylated Liposomal Doxorubicin Using LAL Assays
05:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
工程纳米材料中内毒素的检测是纳米医学领域面临的重大挑战之一。在这里, 我们提出了一个案例研究, 描述了由三种不同的 lal 格式组成的框架, 以估计纳米颗粒中潜在的内毒素污染。
Tags
Endotoxin
Nano-formulations
Limulus Amoebocyte Lysate (LAL) Assays
Calibration Standard
Control Standard Endotoxin
LAL Grade Water
Quality Control
Instrument Settings
Test ID
Data Group
Hardware
LAL Method
Serial Number
System ID
Serial Port
Sample ID
Negative Control
Standard Curve
Test Samples
Inhibition Enhancement Control
Dilution
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