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JoVE Journal Behavior
En Microphysiologic Platform for menneskelige fedt
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En Microphysiologic Platform for menneskelige fedt: klemt inde hvidt fedtvæv

Article DOI: 10.3791/57909-v • 08:14 min • August 15th, 2018
August 15th, 2018 •
1, 2, 2, 2
1Department of Pharmacology, Louisiana State University Health Sciences Center, 2Department of Surgery, Louisiana State University Health Sciences Center

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Hvidt fedtvæv (WAT) er kritisk mangler i dens nuværende primære kultur modeller, hindrer farmakologiske udvikling og metaboliske undersøgelser. Vi præsenterer her, en protokol for at producere et fedt microphysiological system af sandwiching WAT mellem ark af stromale celler. Denne konstruktion giver en stabil og fleksibel platform for primære WAT kultur.

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Adfærd spørgsmålet 138 fedtvæv adipocytter fedme microphysiological systemer organer på chips stof screening udvikling af lægemidler miljømæssige toksicitet primære kultur diabetes stofskifte
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