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Journal / Genetics / 字符串装配 grna 克隆 (stacr)…
JoVE Journal
Genetics

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JoVE Journal Genetics
字符串装配 grna 克隆 (stacr) 的可自定义协议
Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
Journal (Genetics)
Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
Journal (Genetics)
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
 
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字符串装配 grna 克隆 (stacr) 的可自定义协议

Article DOI: 10.3791/58556-v • 10:00 min • December 26th, 2018
December 26th, 2018 •
1,2, 1,2, 3, 3, 2,4, 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center

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在这里, 我们提出了字符串组装 grna 克隆 (stagar), 一种方法, 可以很容易地将 grna 载体的 crispr/cas9 方法。st简便, star 使 grna 多路复用变得简单、高效且可定制。

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遗传学 第142期 crispr casr cas9 dcas9 grna 克隆 grna 多路复用 基因组编辑 转录组编辑
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