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Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
DOI:

10:45 min

June 03, 2019

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Chapters

  • 00:04Title
  • 00:57Cell Segmentation: Generation of a Cell Mask for Time Lapse Movies
  • 02:33Loading the Generated Cell Mask into Surface Manager
  • 03:36Analysis of Actomyosin Pulses in Surface Manager at Local Cellular Scale
  • 04:51Selective Surface Extraction of Curved Epithelial Tissues
  • 08:41Results: Behavior of Myosin II at the Cellular and Tissue Scale in a Drosophila Egg Chamber
  • 10:13Conclusion

Summary

Automatic Translation

This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.

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