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Journal / Developmental Biology / Visualisering cellulære Gibberellin niveauer ved…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Visualisering cellulære Gibberellin niveauer ved hjælp af NlsGPS1 Förster resonans energi overføre (FRET) Biosensor
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Visualisering cellulære Gibberellin niveauer ved hjælp af NlsGPS1 Förster resonans energi overføre (FRET) Biosensor

Article DOI: 10.3791/58739-v • 08:53 min • January 12th, 2019
January 12th, 2019 •
1, 1, 1, 1
1Sainsbury Laboratory, University of Cambridge

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Gibberellin Perception Sensor 1 (GPS1) er den første Förster resonans energi transfer-baseret biosensor til måling af de cellulære niveauer af gibberellin Phytohormoner med en høj spatiotemporelle opløsning. Denne protokol rapporter om metoden til at visualisere og kvantificere cellulære gibberellin niveauer ved hjælp af genetisk kodet nlsGPS1 biosensor i Arabidopsis hypocotyls og roden tips.

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Udviklingsmæssige biologi spørgsmålet 143 planter biokemi Life sciences Life Sciences (generelt) gibberellin Phytohormone Spatiotemporal udvikling af anlægget celle vækst Biosensor FRET
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