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Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice

Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice

DOI:
10.3791/58871-v

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09:50 min

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February 11, 2019

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, , , ,
1Department of Internal Medicine 1, University Erlangen-Nürnberg,University Hospital Erlangen, 2Institute of Pathology, Department of Nephropathology,University Hospital Erlangen, 3Deutsches Zentrum Immuntherapie (DZI),University Hospital Erlangen

Chapters

  • 00:04Title
  • 00:49T Cell-depleted Bone Marrow Reconstitution (Day 1)
  • 03:58T Cell Transfer (Day 2)
  • 05:19Graft Versus Host Disease (GvHD)-related Lesion Scoring
  • 06:46Results: Representative GvHD Scoring
  • 08:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol that describes allogeneic hematopoietic stem cell transplantation and allows repetitive mini-endoscopic evaluations of the distal colon in situ for the presence, characteristics, and severity of colonic inflammation within live mice suffering from intestinal graft-versus-host disease.

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