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Journal / Bioengineering / Live celle analyse av skjæring Stress på…
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Bioengineering

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JoVE Journal Bioengineering
Live celle analyse av skjæring Stress på Pseudomonas aeruginosa bruker en automatisert høyere gjennomstrømming Microfluidic System
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Live celle analyse av skjæring Stress på Pseudomonas aeruginosa bruker en automatisert høyere gjennomstrømming Microfluidic System

Article DOI: 10.3791/58926-v • 09:12 min • January 16th, 2019
January 16th, 2019 •
*1, 1, 1, 1, 2, 2, 3, 1, *4
1Department of Chemistry, Doane University, 2Department of Biology, Doane University, 3Department of Pathology and Microbiology, University of Nebraska Medical Center, 4Department of Physics and Engineering, Doane University
* These authors contributed equally

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Her beskriver vi bruk av en høyere gjennomstrømming microfluidic bioreactor kombinert med fluorescerende mikroskop for analyse av skjæring stress effekter på Pseudomonas aeruginosa biofilm uttrykke grønne fluorescerende proteiner, inkludert instrument sette opp, bestemmelse av biofilm dekning, vekst og morfologiske egenskaper.

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Bioteknologi problemet 143 Pseudomonas aeruginosa forbedret grønne fluorescerende protein microfluidics skråstille biofilm minimal media fluorescens mikroskopi lyse-feltet mikroskopi biofilm dekning bildeanalyser
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