Journal
/
/
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
DOI:

11:58 min

January 30, 2019

, ,

Chapters

  • 00:04Title
  • 00:3132P-labeled Primer Labeling for In Vitro Selective 2‘-hydroxyl Acylation Analyzed by Primer Extension (SHAPE)
  • 03:44Small Molecule Binding and RNA 2‘-OH Modification
  • 06:29Marker Preparation and Primer Extension
  • 08:59PAGE Analysis
  • 10:15Results of In Vitro and In-cell SHAPE for Analyzing Small Molecule Induced Pre-mRNA Structural Changes
  • 11:02Conclusion

Summary

Automatic Translation

Detailed protocols for both in vitro and in-cell selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) experiments to determine the secondary structure of pre-mRNA sequences of interest in the presence of an RNA-targeting small molecule are presented in this article.

Related Videos

Read Article