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Journal / Bioengineering / CRISPR/Cas12a Multiplex Genom Bearbeitung von…
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Bioengineering

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JoVE Journal Bioengineering
CRISPR/Cas12a Multiplex Genom Bearbeitung von Saccharomyces cerevisiae und die Schaffung von Hefe Pixel Kunst
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CRISPR/Cas12a Multiplex Genom Bearbeitung von Saccharomyces cerevisiae und die Schaffung von Hefe Pixel Kunst

Article DOI: 10.3791/59350-v • 10:18 min • May 28th, 2019
May 28th, 2019 •
1,2, 1, 3,4, 1, 1
1DSM Biotechnology Center, 2Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, 3BrisSynBio, University of Bristol, Life Sciences Building, 4School of Biological Sciences, University of Bristol, Life Sciences Building

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Das CRISPR/Cas12a-System in Kombination mit einem einzigen crRNA-Array ermöglicht eine effiziente Multiplex-Bearbeitung des S. cerevisiae Genoms an mehreren Loci gleichzeitig. Dies wird durch die Konstruktion von Carotinoid produzierenden Hefestämmen demonstriert, die anschließend zur Erzeugung von Hefepixelkunst verwendet werden.

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Bioengineering Ausgabe 147 CRISPR/Cas12a CRISPR/Cpf1 CRISPR/Cas9 Multiplex-Genombearbeitung Saccharomyces cerevisiae Hefepixelkunst
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