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Journal / Biochemistry / Nitropeptid Profilierung und Identifikation…
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Biochemistry

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JoVE Journal Biochemistry
Nitropeptid Profilierung und Identifikation illustriert von Angiotensin II
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Nitropeptid Profilierung und Identifikation illustriert von Angiotensin II

Article DOI: 10.3791/59391-v • 07:31 min • June 16th, 2019
June 16th, 2019 •
1,2, 3, 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

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Die proteomische Profilierung von Tyrosin-Nitrat-Proteinen war aufgrund der geringen Häufigkeit der 3-Nitrotytyrosin-Modifikation eine anspruchsvolle Technik. Hier beschreiben wir einen neuartigen Ansatz für die Nitropeptidanreicherung und Profilierung unter Verwendung von Angiotensin II als Modell. Diese Methode kann für andere In-vitro- oder In-vivo-Systeme erweitert werden.

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Biochemie Ausgabe 148 Nitropeptid Angiotensin II Nitrotyrosin Dimethylkennzeichnung Natriumdithionat Biotinkennzeichnung Anreicherung
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