An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

Name: An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants
Uploaded: 2019-11-03T13:00:00+00:00
Duration: 7 min 10 s
Description: Watch this Scientific Journal Video about An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants at JoVE.com

Christian S. Thudium; Amalie Engstrom; Solveig  S. Groen; Morten  A. Karsdal; Anne-Christine Bay-Jensen

doi:10.3791/59467

Journal

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Biology

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An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

JoVE Journal
Biology

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JoVE Journal Biology

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

DOI:

10.3791/59467-v

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07:10 min

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November 03, 2019

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Christian S. Thudium*¹, Amalie Engstrom*^1,2, Solveig S. Groen, Morten A. Karsdal, Anne-Christine Bay-Jensen

¹Nordic Bioscience, ²Department of Biomedical Sciences,University of Copenhagen

Chapters

00:04Title
00:50Tissue Isolation
02:35Bovine Cartilage Explant Treatment and Assessment of Metabolic Activity
04:21Results: Analysis of Cartilage Remodeling in Bovine Knee Explants
06:14Conclusion

Summary

Automatic Translation

Here, we present a protocol describing isolation and culturing of cartilage explants from bovine knees. This method provides an easy and accessible tool to describe tissue changes in response to biological stimuli or novel therapeutics targeting the joint.