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JoVE Journal Neuroscience
Geração de hidrogéis com base em colágeno 3-D para analisar o crescimento e o comportamento axonal durante o desenvolvimento do sistema nervoso
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Geração de hidrogéis com base em colágeno 3-D para analisar o crescimento e o comportamento axonal durante o desenvolvimento do sistema nervoso

Article DOI: 10.3791/59481-v • 09:10 min • June 25th, 2019
June 25th, 2019 •
1,2,3,4, 1,2,3,4
1Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Parc Científic de Barcelona, 2Department of Cell Biology, Physiology, and Immunology, Universitat de Barcelona, 3Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), 4Institute of Neuroscience, University of Barcelona

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Aqui, fornecemos um método para analisar o comportamento dos axônios crescentes em matrizes 3D, imitando seu desenvolvimento natural.

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Neurociência edição 148 culturas 3-D do hidrogel crescimento axonal explants do tecido sistema nervoso embrionário Transfection da pilha chemoattraction chemorepulsion
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