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Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
JoVE Journal
Engineering
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JoVE Journal Engineering
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
DOI:

09:13 min

July 06, 2019

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Chapters

  • 00:04Title
  • 00:58Alignment of the Microscope Spatial Beam Overlap
  • 01:58Alignment of the Microscope Temporal Synchronization of the Beams Use of the Autocorrelator
  • 03:24Alignment of the Microscope Aligning the Femtosecond Laser Beams :OPO and Ti:Sa
  • 04:44Optimization of Temporal Synchronization of Beams
  • 05:48Optimization of Spatial Overlap of Beams – Transmission Image Acquisition of OPO and Ti:Sa
  • 06:48SRS Image Acquisition
  • 07:40Results: Nonlinear Microscope Based on Stimulated Raman Scattering
  • 08:43Conclusion

Summary

Automatic Translation

In this manuscript, the implementation of a stimulated Raman scattering (SRS) microscope, obtained by the integration of an SRS experimental set-up with a laser scanning microscope, is described. The SRS microscope is based on two femtosecond (fs) laser sources, a Ti-Sapphire (Ti:Sa) and synchronized optical parametric oscillator (OPO).

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