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Journal / Biochemistry / Traduire Ribosome Affinity Purification (TRAP)…
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Biochemistry
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JoVE Journal Biochemistry
Traduire Ribosome Affinity Purification (TRAP) pour l'isolement de l'ARN des cellules endothéliales in vivo
Identification of Functional Protein Regions Through Chimeric Protein Construction
Journal (Biochemistry)
Identification of Functional Protein Regions Through Chimeric Protein Construction
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
Journal (Biochemistry)
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
 
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Traduire Ribosome Affinity Purification (TRAP) pour l'isolement de l'ARN des cellules endothéliales in vivo

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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Nous présentons une approche pour purifier l'ARNm ribosome-lié des cellules endothéliales vasculaires (ECs) directement dans le cerveau de souris, poumon et tissus cardiaques par l'intermédiaire de l'étiquette génétique EC-spécifique de la protéine verte améliorée de fluorescence (EGFP) dans des ribosomes en combination avec la purification d'ARN .

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Biochimie Numéro 147 Angiogenèse différenciation artériolaire cellules endothéliales vasculaires traduction de la purification d'affinité de ribosome extraction d'ARN qPCR en temps réel
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