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JoVE Journal Biochemistry
Översätta ribosom affinitetsrening (fälla) för RNA-isolering från endotelceller in vivo
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Översätta ribosom affinitetsrening (fälla) för RNA-isolering från endotelceller in vivo

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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Vi presenterar en metod för att rena ribosombunden mRNA från vaskulära endotelceller (ECs) direkt i mushjärna, lung-och hjärtvävnad via EC-specifika genetiska tag av Enhanced Green fluorescens protein (egfp) i ribosomer i kombination med RNA rening .

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Biokemi angiogenes arteriolär differentiering vaskulära endotelceller översätta Ribosomen affinitetsrening RNA-extraktion realtid qPCR
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