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Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

DOI:
10.3791/59632-v

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10:31 min

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August 15, 2019

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, , , , ,
1Department of Molecular Microbiology and Immunology,Saint Louis University, 2School of Veterinary Medicine University of California Davis

Chapters

  • 00:04Title
  • 01:28Organ Isolation
  • 03:06Zika Virus Focus Forming Assay (FFA)
  • 08:05Results: Viral Replication and Focus Forming Assay
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of the protocol is to demonstrate the techniques used to investigate viral disease by isolating and quantifying Zika virus, from multiple organs in a mouse following infection.

Tags

Zika VirusViral InfectionMouse ModelCentral Nervous SystemOrgan HarvestingViral PathogenesisViral SpreadImmunological ResponseFlavivirusAlphavirusDengue VirusChikungunya VirusViral Quantification

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