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Journal / Neuroscience / 小鼠视网膜冷冻节的制备,用于免疫性植物化学…
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JoVE Journal Neuroscience
小鼠视网膜冷冻节的制备,用于免疫性植物化学
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小鼠视网膜冷冻节的制备,用于免疫性植物化学

Article DOI: 10.3791/59683-v • 05:25 min • July 1st, 2019
July 1st, 2019 •
1, 2, 2, 1
1Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, University of Pennsylvania School of Veterinary Medicine

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本报告介绍了制备冷冻小鼠视网膜部分用于免疫组织化学(IHC)的综合方法。 所述方法包括眼部后杯的解剖、甲醛固定、嵌入最佳切割温度(OCT)介质和组织定向、切片和免疫染色。

Tags

神经科学 问题 149 视网膜 冷冻截断 最佳切割温度介质 OCT 免疫组化学 小鼠眼电
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