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JoVE Journal Cancer Research
Fysiologisk patient afledte 3D Sfæroider til anti-neoplastisk Drug screening for at målrette kræft stamceller
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Fysiologisk patient afledte 3D Sfæroider til anti-neoplastisk Drug screening for at målrette kræft stamceller

Article DOI: 10.3791/59696-v • 10:03 min • July 5th, 2019
July 5th, 2019 •
*1, *1, *1, *2, *1, *2, *2, 1,2,3,4
1Department of Biomedical Engineering, University of Michigan, 2Department of Materials Science and Engineering, University of Michigan, 3Rogel Cancer Center, School of Medicine, University of Michigan, 4Macromolecular Science and Engineering, University of Michigan
* These authors contributed equally

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Denne protokol beskriver generering af patient afledte sfæroider og downstream-analyse, herunder kvantificering af proliferation, cytotoksicitets test, flow cytometri, immunofluorescens farvning og konfokale billeder, med henblik på at vurdere Kandidaternes potentiale som anti-neoplastisk terapi. Denne protokol understøtter præcisionsmedicin med identifikation af specifikke lægemidler for hver patient og sygdomsstadie.

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Kræftforskning patient afledte kræft stamceller Drug screening høj gennemløb hængende dråbe sfæroider præcisionsmedicin
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