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Journal / Genetics / 在Vivo前向遗传屏幕识别新的神经保护基因在果蝇黑色素…
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JoVE Journal Genetics
在Vivo前向遗传屏幕识别新的神经保护基因在果蝇黑色素
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在Vivo前向遗传屏幕识别新的神经保护基因在果蝇黑色素

Article DOI: 10.3791/59720-v • 10:00 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1, 1, 2, 2, 1, 2, 1
1Department of Biological Sciences, University of Alabama, 2Department of Genetics, University of Wisconsin-Madison

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我们提出了一个协议,使用前向遗传方法筛选在果蝇黑色素细胞中表现出神经退化的突变体。它结合了爬升测定、组织分析、基因映射和DNA测序,最终鉴定出与神经保护过程相关的新基因。

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遗传学 问题 149,果蝇黑色素,神经退化 前向遗传屏幕 爬坡测定 成体学 基因图谱 DNA测序
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