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Journal / Medicine / Farklılaşmış HepaRG hücrelerinde veziküler…
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Farklılaşmış HepaRG hücrelerinde veziküler steatozu indükleyen ve karakterize etme
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Farklılaşmış HepaRG hücrelerinde veziküler steatozu indükleyen ve karakterize etme

Article DOI: 10.3791/59843-v • 09:15 min • July 18th, 2019
July 18th, 2019 •
1,2, 3, 3, 3, 2,4, 2,3,5,6, 1,3
1Department of Molecular Medicine, Sapienza University, 2Department of Internal Medicine - DMISM, Sapienza University, 3Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 4Pasteur Institute Italy-Fondazione Cenci Bolognetti, 5INSERM U1052-Cancer Research Center of Lyon, 6Department of Hepatology, Croix Rousse Hospital, Hospices Civils de Lyon

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Bu çalışmada, farklılaştırılmış HepaRG hücrelerinde yağ asidi tuz sodyum oleat ile karaciğer veziküler steatozis inducing için ayrıntılı bir protokol tarif ve tutarlı Anti-Stokes dahil lipid birikiminin tespiti ve ölçülmesi için yöntemler istihdam Raman saçılma (CARS) mikroskobu, sitofluorimetrik analiz, yağ kırmızı O boyama, ve qPCR.

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Tıp sayı 149 HepaRG karaciğer veziküler steatozis NAFLD lipid damlacıkları CARS microkopi
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