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Journal / Immunology and Infection / Bruk av en influensa antigen Microarray å måle…
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Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Bruk av en influensa antigen Microarray å måle bredden av serum antistoffer over virus under typer
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Bruk av en influensa antigen Microarray å måle bredden av serum antistoffer over virus under typer

Article DOI: 10.3791/59973-v • 08:52 min • July 26th, 2019
July 26th, 2019 •
1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2
1Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology, University of California Irvine

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Vi presenterer en protokoll for bruk av et protein Microarray konstruert ved å skrive ut influensa antigener på nitrocellulose-belagte lysbilder å samtidig sonde serum for flere antistoff isotypes mot over 250 antigener fra ulike virusstammer, og dermed som tillater måling av bredden av serum antistoffer på tvers av virus under typer.

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Immunologi og infeksjon protein Microarray influensa virus antigen antistoff Hemagglutinin immunitet
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