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Immunology and Infection

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JoVE Journal Immunology and Infection
Expression of Functional rekombinant hemagglutinin og neuraminidase Proteiner fra Novel H7N9 Influensa virus Bruke Baculovirus Expression System
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Expression of Functional rekombinant hemagglutinin og neuraminidase Proteiner fra Novel H7N9 Influensa virus Bruke Baculovirus Expression System

Article DOI: 10.3791/51112-v • 12:18 min • November 6th, 2013
November 6th, 2013 •
1,2, 1,3, 1
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Medicine, Icahn School of Medicine at Mount Sinai

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Her beskriver vi en måte å uttrykke korrekt foldet og funksjonelle influensavirus overflateantigener avledet fra romanen kinesiske H7N9-viruset i insektceller. Teknikken kan tilpasses til å uttrykke ectodomains av noen virale eller cellulære overflateproteiner.

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Infeksjon Influensa A-viruset, Influensa Human influensa i Birds influensavaksiner hemagglutinin neuraminidase H7N9 baculovirus insektceller rekombinant protein uttrykk
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