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Journal / Biochemistry / En tilpasselig tilgang til enzymatisk produktion…
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Biochemistry

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JoVE Journal Biochemistry
En tilpasselig tilgang til enzymatisk produktion og rensning af Diterpenoid naturprodukter
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
Journal (Biochemistry)
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En tilpasselig tilgang til enzymatisk produktion og rensning af Diterpenoid naturprodukter

Article DOI: 10.3791/59992-v • 07:59 min • October 4th, 2019
October 4th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Plant Biology, University of California Davis

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Her præsenterer vi let at bruge protokoller til fremstilling og rensning af isomere metabolitter gennem kombinatorisk ekspression af biosyntetiske enzymer i Escherichia coli eller Nicotiana benthamiana, efterfulgt af kromatografisk produkt Rensning. De resulterende metabolitter er egnede til forskellige undersøgelser, herunder molekylær struktur karakterisering, enzym funktionelle undersøgelser, og Bioaktivitet assays.

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Biokemi isomere biosyntese co-Expression diterpenforbindelser syntase cytochrom P450 monooxygenase Nicotiana benthamiana Zea mays plante specialiseret metabolisme
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